Pruebas
SRY-Box Transcription Factor 10 Measurement
The determination of the amount of SRY-box transcription factor 10 in a sample.
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Pruebas
The determination of the amount of SRY-box transcription factor 10 in a sample.
Technology
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Glossary Term
A two-step setup in which RNA is first copied into complementary DNA by a reverse transcriptase and then amplified by PCR. Used for RNA viruses such as influenza, RSV, SARS-CoV-2, and HCV. In procurement documents, confirm whether "RT-PCR" refers to real-time (quantitative) or endpoint (qualitative) reading.
Glossary Term
The action of copying RNA into DNA by reverse transcriptase.
Glossary Term
A genetic process in which single-stranded RNA with base sequence complementary to a region of plus strand DNA is synthesized by DNA-dependent RNA polymerases. Following the binding of RNA polymerase on DNA promoter regions, the RNA product is synthesized in a 5' to 3' direction, elongating RNA along the DNA strand as the polymerase proceeds, accompanied by unwinding and rewinding of the DNA template.
Artículo
Research record from Clin Chim Acta, 2026-06-09. PMID 42263803.
Artículo
Research record from Diagn Microbiol Infect Dis, 2026-08-26. PMID 42700662.
Artículo
Research record from Trends Biotechnol, 2026-05-14. PMID 42135078.
Artículo
Research record from J Gen Virol, 2026-10-01. PMID 42842305.
Technology
A molecular genetic technique that isolates and sequences chromosomal regions that are rich in open chromatin. First, nuclei are harvested from a cellular sample. Then a hyperactive Tn5 transposase is added to the nuclei where it excises non-nucleosomal DNA strands and ligates co-administered high-throughput sequencing adapters (tagmentation). The tagged DNA fragments are isolated, amplified by PCR and sequenced. The number of reads for specific region of DNA correlate with increased chromatin accessibility and this method can identify regions of transcription factor and nucleosome binding.
Technology
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse transcriptase.
Indicadores
This gene plays a role in the transcriptional activation of androgen responsive genes.