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Technology
Selection and deposition of individual cells of a particular phenotype from a mixed population into a separate tube or tissue culture plate by the use of a fluorescence-activated cell sorter (FACS) and fluorescently-labeled antibodies specific for surface molecules on the cells to be sorted.
Glossary Term
Fluorescence is a luminescence (i.e., optical phenomenon) in cold bodies, in which a molecule absorbs a high-energy photon, and re-emits it as a lower-energy (longer-wavelength) photon. The energy difference between the absorbed and emitted photons ends up as molecular vibrations (heat).
Technology
A physical mapping approach that uses fluorescent tags to detect hybridization of probes within metaphase chromosomes or less condensed somatic interphase chromatin. This technique can be used for identification of chromosomal abnormalities and for gene mapping.
Technology
A technique in which samples that either contain fluorescent compounds or are stained with fluorescent dyes are exposed to a wavelength of light that excites the fluorophore, allowing for an image to be captured by a microscope.
Technology
An immunoassay in which a fluorescent tracer is used to detect an analyte of interest by triggering the emission of photons when subjected to polarized light.
Technology
A process to measure the proximity of two fluorochromes whereby the excitation energy of one fluorochrome is released and absorbed by the second fluorochrome. It is used to study interactions and structure of macromolecules. (NCI)
Technology
Fluorescent spectroscopy or fluorometry is a type of electromagnetic spectroscopy used for analyzing fluorescent spectra. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light of a lower energy, typically, but not necessarily, visible light.
Artículo
Research record from Anal Chim Acta, 2026-04-22. PMID 42191311.
Artículo
Research record from J Fluoresc, 2026-06-30. PMID 42377648.
Artículo
Research record from Life (Basel), 2026-05-16. PMID 42195383.
Artículo
Research record from Microorganisms, 2026-03-09. PMID 41900363.
Artículo
Research record from Heart Lung, 2026-10-07. PMID 42843109.
