Technology
Immunoaffinity Chromatography
Affinity chromatography using monoclonal or polyclonal antibodies, or (less commonly) antigens, as the immobilized binding phase.
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Technology
Affinity chromatography using monoclonal or polyclonal antibodies, or (less commonly) antigens, as the immobilized binding phase.
Technology
A chromatographic separation technique based on the ability of proteins to bind specific ligands reversibly.
Glossary Term
The strength of noncovalent chemical binding between two substances as measured by the dissociation constant of the complex.
Article
Research record from J Proteomics, 2026-09-19. PMID 42762864.
Article
Research record from Ecotoxicol Environ Saf, 2026-09-14. PMID 42735481.
Article
Research record from J Med Chem, 2026-04-04. PMID 41934649.
Indicators
An immunoglobulin isotype (subclass). This isotype has the unique ability to bind with high affinity to IgE receptors (FceRI) on mast cells and basophils and induce degranulation and cytokine production by these cells when they are crosslinked by antigen. In addition to a central role in atopy and allergic responses, it has been speculated that IgE-mediated mast cell degranulation, by its release of mediators that increase vascular permeability, has an important role in the initiation of immune response in general.
Indicators
The most abundant immunoglobulin G (IgG) subclass in the circulation of healthy individuals. The IgG1 core hinge sequence contains 15 amino acids and two interchain disulfide bonds. This subtype has high affinity for binding both Fc receptors and complement component C1q. IgG1 is the predominant antibody subclass that responds to antigens derived from both soluble and membrane proteins. Reduced levels of circulating IgG1 can indicate the presence of a variety of primary and secondary antibody deficiencies, which can result in hypogammaglobulinemia.
Indicators
The second most abundant immunoglobulin G (IgG) subclass in the circulation of healthy individuals. The IgG2 core hinge sequence contains 12 amino acids and up to four interchain disulfide bonds. This subtype has low affinity for binding both Fc receptors and complement component C1q. The production of this IgG2 is stimulated by exposure to bacterial capsular polysaccharides and other antigenic glycans.
Indicators
Along with the immunoglobulin G4 subclass (IgG4), the IgG3 subtype makes up a minor part of circulating antibodies in healthy individuals. The core hinge sequence contains approximately four times the number of amino acids as the other subclasses; allotypes of IgG3 can have up to 62 hinge residues and up to four interchain disulfide bonds. The increased hinge size for IgG3 increases the separation between the Fc (constant fragment) tail and Fab (antigen binding fragment) arms of antibodies of this subclass. This enhances the relative flexibility of the molecule, which may modulate antigen-binding capacity and immune complex formation, and may increase the affinity for binding to both Fc receptors and complement component C1q.
Indicators
Along with the immunoglobulin G3 subclass (IgG3), the IgG4 subtype makes up a minor part of circulating antibodies in healthy individuals. The IgG4 core hinge sequence contains 12 amino acids and two interchain disulfide bonds.This subtype has moderate affinity for binding Fc receptors and does not bind complement component C1q. The production of IgG4 can be stimulated following repeated or long-term exposure to antigen. Elevated levels are found in various diseases and infections and can be indicative of an fibroinflammatory syndrome known as IgG4-related disease.
Indicators
The major immunoglobulin secreted during a primary immune response. IgM binds with low affinity but high avidity (multiple binding sites) because it not only occurs as monomers but also as pentamers and hexamers.