Technology
An enhancement of immunoelectrophoresis in which a series of post-electrophoretic gel slabs are layered with cellulose-acetate gels saturated with specific antibodies. The resulting antigen-antibody complexes fixed on the second gel may then be stained, allowing sensitive and specific qualitative visual identification of paraproteins by electrophoretic position. It can be used to identify specific proteins in the blood or urine. It has greatest application in the identification and monitoring of monoclonal proteins that are produced in conditions like Waldenstom's macroglobulinaemia and multiple myeloma.
Diagnostic Methods
Technology
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
Diagnostic Methods
Technology
An imaging technique that uses multiple photons to absorb infrared light, thereby minimizing phototoxicty and photobleaching, making it an ideal method for imaging living specimens, especially those involving deep tissue.
Diagnostic Methods
Technology
A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Diagnostic Methods
Article
Research record from Se Pu, 2026-03-01. PMID 41814896.
Article
Research record from Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2026-08-01. PMID 42802440.
Article
Research record from J Med Microbiol, 2026-04-01. PMID 41961530.
Article
Research record from Vet Res Commun, 2026-06-22. PMID 42329486.
Article
Research record from J Liq Biopsy, 2026-10-06. PMID 42893057.
Article
Research record from J Clin Microbiol, 2026-09-16. PMID 42747277.
Article
Research record from J Thromb Haemost, 2026-03-04. PMID 41791653.
Glossary Term
Alternate Splicing is a process in which multiple protein isoforms or products are generated from a single gene as a result of splicing together different nonconsecutive exons during the processing of initially identical nascent transcripts of a gene into mature mRNA transcripts. An exon may be connected to any one of several alternative exons to form the mRNA and generate related proteins, often in a tissue or developmental stage specific manner.
Pcr Terms
