National Cancer Institute (source definition)
Loop-mediated Isothermal Amplification
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-19
professional use
References
- NCI Thesaurus 26.09d — Loop-mediated Isothermal Amplification (C102661)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C102661. NCI definition, reproduced without modification under CC BY 4.0.
- Development of loop-mediated isothermal amplification and recombinase polymerase amplification combined with lateral flow dipstick assay for the rapid detection of Salmon gill poxvirus. — J Virol Methods, 2026-10-08 (PMID 42849817; literature)
- Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification. — Emerg Infect Dis, 2026-10-01 (PMID 42811284; literature)
- A cationic platinum nanocluster-based loop-mediated isothermal amplification platform with pretreatment by magnetic nanoparticles for colorimetric and quantitative detection of MRSA from clinical samples. — Analyst, 2026-09-29 (PMID 42689457; literature)
- A solid-state heater-imager for quantitative evaluation of colorimetric isothermal nucleic acid amplification on paper. — Lab Chip, 2026-09-16 (PMID 42578606; literature)
What it is
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C102661. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.