National Cancer Institute (source definition)
Immunofixation
An enhancement of immunoelectrophoresis in which a series of post-electrophoretic gel slabs are layered with cellulose-acetate gels saturated with specific antibodies. The resulting antigen-antibody complexes fixed on the second gel may then be stained, allowing sensitive and specific qualitative visual identification of paraproteins by electrophoretic position. It can be used to identify specific proteins in the blood or urine. It has greatest application in the identification and monitoring of monoclonal proteins that are produced in conditions like Waldenstom's macroglobulinaemia and multiple myeloma.
Review and references
Automated source check (NCI definition)
2026-10-20
2026-10-24
professional use
References
- NCI Thesaurus 26.09d — Immunofixation (C54536)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C54536. NCI definition, reproduced without modification under CC BY 4.0.
- Plasma Cell Leukemia with A "Missing" Monoclonal Protein on Immunofixation. — Clin Chem, 2026-09-01 (PMID 42690175; literature)
- Commentary on Plasma Cell Leukemia with a "Missing" Monoclonal Protein on Immunofixation. — Clin Chem, 2026-09-01 (PMID 42690174; literature)
- Commentary on Plasma Cell Leukemia with a "Missing" Monoclonal Protein on Immunofixation. — Clin Chem, 2026-09-01 (PMID 42690173; literature)
What it is
An enhancement of immunoelectrophoresis in which a series of post-electrophoretic gel slabs are layered with cellulose-acetate gels saturated with specific antibodies. The resulting antigen-antibody complexes fixed on the second gel may then be stained, allowing sensitive and specific qualitative visual identification of paraproteins by electrophoretic position. It can be used to identify specific proteins in the blood or urine. It has greatest application in the identification and monitoring of monoclonal proteins that are produced in conditions like Waldenstom's macroglobulinaemia and multiple myeloma.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C54536. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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