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Glossary Term
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Tests
The determination of the amount of Dientamoeba fragilis DNA in a biological sample.
Glossary Term
Drug-, radiation-induced, or spontaneous injuries to DNA that introduce deviations from its normal double-helical conformation. These changes include structural distortions that interfere with replication and transcription, as well as point mutations that disrupt base pairs and exert damaging effects on future generations through changes in DNA sequence. If the damage is minor, it can often be repaired (DNA repair); extensive damage can induce apoptosis.
Tests
A determination of the amount of DNA demethylation pathway-related mRNA in a biological sample.
Glossary Term
Any method that extracts and purifies DNA from biological samples, which can include the proper handling of biological samples prior to nucleic acid isolation, the separation of DNA from the rest of the components in the samples, and the storage of the DNA isolates.
Glossary Term
A family of enzymes that catalyze the formation of phosphodiester bonds to repair broken strands of DNA.
Glossary Term
Enzymatic restoration of DNA structure after chemical, radiation, or spontaneous damage.
Tests
The determination of the amount of Dnase-B antibody present in a sample.
Tests
The determination of the amount of Entamoeba histolytica DNA present in a sample.
Tests
The determination of the amount of enteropathogenic Escherichia coli eae DNA present in a sample.
Tests
The determination of the amount of enterotoxigenic Escherichia coli LtA, ST1a and/or ST1b DNA present in a sample.
Tests
The determination of the amount of Escherichia coli DNA in a biological sample.
