Technology
Silver Staining Method
Silver Staining or GMS staining is a technique for demonstrating fungi in tissue sections. It is primarily based on staining the polysaccharides in fungal cell wall (From BioGenex).
Loading
Loading
Search products, companies, tests, and guides — go from query to quote request in one place.
Technology
Silver Staining or GMS staining is a technique for demonstrating fungi in tissue sections. It is primarily based on staining the polysaccharides in fungal cell wall (From BioGenex).
Technology
A rapid, simple, visual and sensitive technique for measuring DNA breakage in individual mammalian cells in response to a test agent. A cell is immobilized in agarose and made permeable and the broken DNA migrates out under the influence of the electric field. A damaged cell has the appearance of a comet with a brightly fluorescent cell body and a tail whose length and fluorescence intensity are related to the number of DNA-strand breaks induced by the test agent.
Technology
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an amplified nucleotide sample that was derived from a single cell.
Technology
Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis is a procedure for estimating molecular weights of polypeptides, and uses SDS, a detergent that dissociates and unfolds oligomeric proteins into its subunits. The SDS binds to the polypeptides to form complexes with fairly constant charge to mass ratios. The electrophoretic migration rate through a gel is therefore determined only by the size of the complexes.
Technology
A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.
Technology
A novel karyotyping procedure which is based on the simultaneous hybridization of 24 chromosome-specific painting probes labeled with different fluorochromes or fluorochrome combinations. The measurement of defined emission spectra by means of interferometer-based spectral imaging allows for the definitive discernment of all human chromosomes in different colors. (from Schrock et al., Hum Genet 1997:101:255-62)
Technology
A laboratory test that produces a quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.
Technology
A procedure used to demonstrate nucleotide sequence variations that occur in the same region of DNA between individuals within a species, or between alleles in an individual to detect mutations in various genes, such as oncogenes, tumor suppressor genes, or genes responsible for genetic diseases. The method detects different conformations adopted by partially denatured DNA fragments resulting from their specific nucleotide sequences under denaturing conditions. Denatured single-stranded DNA fragments are allowed to partially renature without formation of double-stranded DNA. The particular conformations adopted by each of the renatured fragments depend on each of their specific nucleotide sequences. The fragments are run on polyacrylamide gels to detect subtle changes in migration due to their particular secondary structures. Resulting fragment band in the gel will misalign if any point mutations are present to alter the conformation.
Technology
An isothermal, in vitro nucleic acid amplification method that allows primer-directed amplification of a target sequence. It utilizes a primer that can be nicked to create a polymerase start site and a DNA polymerase that will displace a downstream strand as it tracks along the template.
Technology
A quantum optical-electrical phenomena consisting of an alteration in light reflectance as a result of binding of molecules to a surface from which total internal reflection is occurring. Used to detect macromolecular interactions, SPR can be used as the basis for a sensor which is capable of sensitive and quantitative measurement of a broad spectrum of chemical and biological entities.
Technology
A method for determining the mass-to-charge ratio (m/z) of gas-phase ions based on measuring how quickly they travel a known distance in an electric field of known strength.
Technology
A potency unit defined as a minimal dose of infectious material at which preparation causes cytopathic effects (changes in the morphology and metabolism of tissue culture cells, indicating cell death, due to suspected infection) in the 50% of the tissue culture-containing flasks inoculated with that dilution of infectious material in the product potency assay or pathogen activity assay.