National Cancer Institute (source definition)
Strand Displacement Amplification
An isothermal, in vitro nucleic acid amplification method that allows primer-directed amplification of a target sequence. It utilizes a primer that can be nicked to create a polymerase start site and a DNA polymerase that will displace a downstream strand as it tracks along the template.
Review and references
Automated source check (NCI definition)
2026-10-20
2026-10-27
professional use
References
- NCI Thesaurus 26.09d — Strand Displacement Amplification (C92626)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C92626. NCI definition, reproduced without modification under CC BY 4.0.
- Dual-tetrahedral DNA-enabled strand displacement amplification biosensor for cell imaging and clinical detection of miR-34a-5p in NAFLD. — Anal Bioanal Chem, 2026-09-29 (PMID 42811206; literature)
- Exponential strand displacement amplification-assisted microchip electrophoresis for rapid and simultaneous detection of base excision repair enzymes. — J Chromatogr A, 2026-09-23 (PMID 42815109; literature)
- Label-free CRISPR/Cas12a biosensor based on G-triplex/Thioflavin T and GlaI-assisted strand displacement amplification for ultrasensitive DNA methylation detection. — Talanta, 2026-06-15 (PMID 42296867; literature)
- An ultrasensitive CRISPR-strand displacement amplification biosensor achieves piRNA-54265 detection and imaging in colorectal cancer cells. — Anal Bioanal Chem, 2026-05-13 (PMID 42120643; literature)
- Isothermal Strand Displacement Amplification for Chemical Sensing: From Molecular Design to Integrated and Intelligent Platforms. — Biosensors (Basel), 2026-10-10 (PMID 42930116; literature)
What it is
An isothermal, in vitro nucleic acid amplification method that allows primer-directed amplification of a target sequence. It utilizes a primer that can be nicked to create a polymerase start site and a DNA polymerase that will displace a downstream strand as it tracks along the template.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C92626. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
Explore more
Same category
Tissue Culture Infectious Dose 50%
A potency unit defined as a minimal dose of infectious material at which preparation causes cytopathic effects (changes in the morphology and metabolism of tissue culture cells, indicatin…
Same categoryEpsilometry
A method to determine microbial susceptibility to antibiotics in which a plastic strip impregnated with the antibiotic of interest is placed on an agar plate that has been inoculated with…
Same categoryViral Identification
A diagnostic procedure to identify and characterize a viral organism.