Glossary Term
Unique Molecular Identifier
PCR TermsEN
Definition
Short random nucleotide sequences that are ligated to each primer molecule during amplification of a nucleotide sample during library preparation to act as a unique tag. Their use helps to identify PCR duplicates in sequencing data.
Extended Notes
Source: National Cancer Institute, NCI Thesaurus 26.09d, C201932. NCI definition, reproduced without modification under CC BY 4.0. Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.
Also Known As
Random BarcodeUMIUnique Molecular Identifier
References
- NCI Thesaurus 26.09d — Unique Molecular Identifier (C201932)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C201932. NCI definition, reproduced without modification under CC BY 4.0.
- The Effect of Unique Molecular Identifier Family Size Using Tumor-Informed Circulating Tumor DNA Analysis in Childhood Cancers. — J Mol Diagn, 2026-09-11 (PMID 42727690; literature)
- Amplification Optimized and Unique Molecular Identifier Guided High Accuracy Full-length CircRNA Sequencing. — Genomics Proteomics Bioinformatics, 2026-01-28 (PMID 41649452; literature)