Glossary Term

Probe

General term for a piece of DNA or RNA corresponding to a gene or sequence of interest, that has been labelled either radioactively or with some other detectable molecule, such as biotin, digoxygenin or fluorescein. As stretches of DNA or RNA with complementary sequences will hybridize, a probe will label viral plaques, bacterial colonies or bands on a gel that contain the gene of interest.

Producto

INFORM Kappa Probe

Technology

Multiplex Ligation-dependent Probe Amplification

A type of high-throughput, multiplex PCR method used to determine abnormal DNA copy number changes of up to 50 genomic DNA or RNA sequences with a single pair of PCR primers. It is frequently used in the detection of genetic aberrations in tumors, and can distinguish sequences differing in only a single nucleotide.

Artículo

Clinical evaluation of probe capture based targeted next generation sequencing for pulmonary infection in immunocompromised patients: a cross-sectional diagnostic accuracy study.

Research record from Infect Dis (Lond), 2026-04-09. PMID 41955630.

Artículo

Comparative Costs and Diagnostic Yields of Next-Generation Sequencing Versus Muscle Biopsy for Multiplex Ligation-Dependent Probe Amplification-Negative Duchenne Muscular Dystrophy.

Research record from Value Health Reg Issues, 2026-05-29. PMID 42216940.

Artículo

Detection and characterization of PRRSV-2 viruses with mutations in binding sites of common diagnostic real-time RT-PCR primer and probe sequences.

Research record from J Virol Methods, 2026-10-21. PMID 42906759.

Artículo

Molecular Evaluation of Rifampicin and Isoniazid Resistance in Pulmonary Tuberculosis Using Cartridge-Based Nucleic Acid Amplification Test and Line Probe Assay.

Research record from Cureus, 2026-06-04. PMID 42403861.

Artículo

Optimisation and analytical assessment of a TaqMan™ probe-based real-time PCR assay designed to diagnose infection with Schistosoma japonicum.

Research record from Parasit Vectors, 2026-06-29. PMID 42374591.

Artículo

Validation of Sequence-Specific Oligonucleotide Probe-Based HLA Homozygosity Classification Using 11-Locus Next-Generation Sequencing in Korean Cord Blood Units: Extended Haplotype Architecture and Implications for Induced Pluripotent Stem Cell Haplobanking.

Research record from Ann Lab Med, 2026-08-28. PMID 42661591.

Technology

Atomic Force Microscopy

A technique utilizing a scanning probe to image and analyze the surface of a material with atomic-level resolution.

Technology

Atomic Resolution Microscopy

Several related technologies for imaging and measuring surfaces on a fine scale, down to the level of molecules and groups of atoms. These technologies share the concept of scanning an extremely sharp tip (3-50 nm radius of curvature) across the object surface. The tip is mounted on a flexible cantilever, allowing the tip to follow the surface profile. When the tip moves in proximity to the investigated object, forces of interaction between the tip and the surface influence the movement of the cantilever. Various interactions can be studied depending on the specific mechanics of the probe. Specialized sensors detect and record the movements of the cantilever, providing an image with resolution at the atomic level.

Technology

Chromogenic In Situ Hybridization

A laboratory procedure that combines the sequence-specific detection aspects of FISH with the simple detection methods of immunohistochemistry. Briefly, sequence-specific probes are hybridized to cells fixed on slides and the sequestered probe is visualized by a peroxidase reaction. It has the advantage of being a less costly assay that does not require fluorescence microscopy with results that are stable over time.