Glossary Term
DNA Ligase
A family of enzymes that catalyze the formation of phosphodiester bonds to repair broken strands of DNA.
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Glossary Term
A family of enzymes that catalyze the formation of phosphodiester bonds to repair broken strands of DNA.
Glossary Term
Major class of enzymes that catalyze the linking together of two molecules (EC class 6) for example DNA ligases that link two fragments of DNA by forming a phosphodiester bond.
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Technology
A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.