Pruebas

DNA Demethylation Pathway mRNA Measurement

A determination of the amount of DNA demethylation pathway-related mRNA in a biological sample.

Producto

ACCURUN 325 Series 200 HBV DNA Positive Control

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ACCURUN 325 Series 700 HBV DNA Positive Control

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ACCURUN 350 CMV DNA Positive Control

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ACCURUN 372 Series 400 HPV DNA Positive Control

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ACCURUN 378 Series 5000 HPV DNA Positive Control

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ACCURUN 872 HPV DNA Negative Control

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ACCURUN 873 HPV DNA Negative Control

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ACCURUN BKV DNA Positive Control

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ACCURUN EBV DNA Positive Control

Technology

Anchored Multiplex Polymerase Chain Reaction

A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.

Technology

Array-based Comparative Genomic Hybridization

A molecular cytogenetic method combining chromosomal comparative genomic hybridization technique and microarray to detect chromosomal aberrations and changes in DNA copy numbers in the genome, using oligonucleotides immobilized on a solid support as targets for hybridization.