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Technology
A procedure used in mass spectrometry analysis. Means of delivering nonvolatile analytes into the gas phase for mass analysis. Best used on molecules which are charged in solution or readily accept a charge. Particularly well suited to the analysis of biomolecules. Can be used to probe tertiary structure and non-covalent interactions (from NCSU Mass Spectrometry Facility web site)
Technology
Selection and deposition of individual cells of a particular phenotype from a mixed population into a separate tube or tissue culture plate by the use of a fluorescence-activated cell sorter (FACS) and fluorescently-labeled antibodies specific for surface molecules on the cells to be sorted.
Technology
A physical mapping approach that uses fluorescent tags to detect hybridization of probes within metaphase chromosomes or less condensed somatic interphase chromatin. This technique can be used for identification of chromosomal abnormalities and for gene mapping.
Technology
An immunoassay in which a fluorescent tracer is used to detect an analyte of interest by triggering the emission of photons when subjected to polarized light.
Technology
A type of gel electrophoresis in which charged molecules travel through a pH gradient. The molecules will come to stop at the point where the net charge on the molecule is equal to that of the surrounding gel.
Technology
Light Microscopy is a technique for producing visible images of structures or details which are too small to be otherwise seen by the human eye. It involves passing light transmitted through or reflected from the subject through a series of lenses to be detected directly by the eye, imaged on a photographic plate, or captured digitally.
Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A quantum optical-electrical phenomena consisting of an alteration in light reflectance as a result of binding of molecules to a surface from which total internal reflection is occurring. Used to detect macromolecular interactions, SPR can be used as the basis for a sensor which is capable of sensitive and quantitative measurement of a broad spectrum of chemical and biological entities.
Technology
A method for determining the mass-to-charge ratio (m/z) of gas-phase ions based on measuring how quickly they travel a known distance in an electric field of known strength.
