Technology
Gel electrophoresis is a method of separating large molecules (such as DNA fragments or proteins) from a mixture of similar molecules by electrophoretic filtration through a gelatinous matrix. Subject to an electric field, each molecule in the sample mixture migrates through the matrix at a different rate, depending mainly on electrical charge and size, resulting in spatial separation within the matrix of each molecular species in the sample mixture. Agarose and acrylamide in a cylindrical tube or slab gel are commonly used media for electrophoresis of proteins and nucleic acids.
Diagnostic Methods
Technology
Use of a DNA or RNA probe to detect the presence of complementary sequences in chromosomes, cells, or tissues.
Diagnostic Methods
Technology
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
Diagnostic Methods
Technology
An analysis done to detect monoallelic loss of genes or DNA regions in a diploid cell.
Diagnostic Methods
Technology
An analysis of DNA or protein to detect the presence and/or location of methyl groups.
Diagnostic Methods
Technology
A type of high-throughput, multiplex PCR method used to determine abnormal DNA copy number changes of up to 50 genomic DNA or RNA sequences with a single pair of PCR primers. It is frequently used in the detection of genetic aberrations in tumors, and can distinguish sequences differing in only a single nucleotide.
Diagnostic Methods
Technology
A proprietary next-generation DNA sequencing technology from Oxford Nanopore Technologies that can directly identify and sequence a DNA molecule as it passes through a nanopore, driven by electrophoresis.
Diagnostic Methods
Technology
Technologies that facilitate the rapid determination of the nucleotide sequence of large numbers of strands or segments of DNA or RNA.
Diagnostic Methods
Technology
A technique that determines the nucleotide sequence of a pre-specified region of DNA or RNA by using primers that are specific for that region.
Diagnostic Methods
Technology
A common molecular biology procedure for detection of RNA molecules. RNA extracted from a sample was denatured and separated by slab gel electrophoresis, then blotted on to a nitrocellulose or nylon sheet and hybridized with labeled DNA or RNA that is complementary to the desired RNA, whose presence is subsequently indicated by autoradiography or other visualization techniques.
Diagnostic Methods
Technology
A DNA sequencing strategy where a single read is initiated from each end of a DNA fragment.
Diagnostic Methods
Technology
A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Diagnostic Methods
