GuideDiagnostic Laboratory Methods

Rapid Amplification of cDNA Ends

A procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3'- or 5'-end of the mRNA.

Review and references

Author

National Cancer Institute (source definition)

Reviewer

Automated source check (NCI definition)

Published

2026-10-20

Updated

2026-10-22

Disclaimer

professional use

References

NCI Thesaurus 26.09d — Rapid Amplification of cDNA Ends (C180698)
Source: National Cancer Institute, NCI Thesaurus 26.09d, C180698. NCI definition, reproduced without modification under CC BY 4.0.
5' Rapid Amplification of cDNA Ends (5' RACE)-Based Targeted RNA Sequencing Assay for the Analysis of Clinically Relevant Alterations in Lung Cancer Patients. — Int J Mol Sci, 2026-08-13 (PMID 42653230; literature)

What it is

A procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3'- or 5'-end of the mRNA.

Source: National Cancer Institute, NCI Thesaurus 26.09d, C180698. NCI definition, reproduced without modification under CC BY 4.0.

Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.

Compliance note

Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.

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