National Cancer Institute (source definition)
High Performance Liquid Chromatography
Frequently referred to simply as HPLC, this form of column chromatography is used frequently in biochemistry. The analyte is forced through a column by liquid at high pressure, which decreases the time the separated components remain on the stationary phase. Less time on the column then translates to narrower peaks in the resulting chromatogram and thence to better selectivity and sensitivity.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-31
professional use
References
- NCI Thesaurus 26.09d — High Performance Liquid Chromatography (C16434)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C16434. NCI definition, reproduced without modification under CC BY 4.0.
- One Health prescribing - development of enantioselective liquid chromatography - tandem mass spectrometry methods to inform chiral switch of prescription pharmaceuticals. — Anal Methods, 2026-10-15 (PMID 42703855; literature)
- Using High-Performance Liquid Chromatography to Study Alterations in Biogenic Amines in Drosophila Models of Brain Disorders. — J Vis Exp, 2026-10-27 (PMID 42949131; literature)
What it is
Frequently referred to simply as HPLC, this form of column chromatography is used frequently in biochemistry. The analyte is forced through a column by liquid at high pressure, which decreases the time the separated components remain on the stationary phase. Less time on the column then translates to narrower peaks in the resulting chromatogram and thence to better selectivity and sensitivity.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C16434. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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