National Cancer Institute (source definition)
Enzyme Multiplied Immunoassay Technique
A type of homogenous immunoassay in which sample is applied to a reagent mixture of enzyme-labeled ligand, antibody, and substrate. Substrate binding to enzyme leads to inactivation and unlabeled-ligand in the sample can be quantified by colorimetric analysis.
Review and references
Automated source check (NCI definition)
2026-10-20
2026-10-22
professional use
References
- NCI Thesaurus 26.09d — Enzyme Multiplied Immunoassay Technique (C111196)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C111196. NCI definition, reproduced without modification under CC BY 4.0.
- Assessing Diazinon Exposure: A GC-MS/MS Validation Study of BChE Measurement by Point-of-Care Testing and Enzyme Multiplied Immunoassay Technique. — Molecules, 2025-05-29 (PMID 40509267; literature)
- Comparison of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and enzyme-multiplied immunoassay technique (EMIT) for quantification of voriconazole plasma concentration from Chinese patients. — Heliyon, 2023-11-13 (PMID 38045154; literature)
What it is
A type of homogenous immunoassay in which sample is applied to a reagent mixture of enzyme-labeled ligand, antibody, and substrate. Substrate binding to enzyme leads to inactivation and unlabeled-ligand in the sample can be quantified by colorimetric analysis.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C111196. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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