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GuideDiagnostic Laboratory Methods

Competitive Enzyme-linked Immunosorbent Assay

A type of ELISA assay that uses a reference antigen to competitively bind labeled antibody against an antigen of interest. The strength of the signal interference caused by antibody bound to the reference antigen is used to determine the concentration of the antigen of interest.

Topic snapshot

Use this page to compare workflow fit, validation burden, and procurement implications before moving into product-level evaluation.

Updated
2026-10-20
Author
National Cancer Institute (source definition)
Reviewer
Automated source check (NCI definition)

Review and references

Author

National Cancer Institute (source definition)

Reviewer

Automated source check (NCI definition)

Published

2026-10-20

Updated

2026-10-20

Disclaimer

professional use

References

What it is

A type of ELISA assay that uses a reference antigen to competitively bind labeled antibody against an antigen of interest. The strength of the signal interference caused by antibody bound to the reference antigen is used to determine the concentration of the antigen of interest.

Source: National Cancer Institute, NCI Thesaurus 26.09d, C178028. NCI definition, reproduced without modification under CC BY 4.0.

Source identity and definition checked automatically on 2026-10-20. No individual clinical interpretation or human reviewer is asserted.

Compliance note

Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.