GuideDiagnostic Laboratory Methods

ELISA

A highly sensitive technique for detecting and measuring antigens or antibodies in a solution; the solution is run over a surface to which immobilized antibodies specific to the substance have been attached, and if the substance is present, it will bind to the antibody layer, and its presence is verified and visualized with an application of antibodies that have been tagged in some way. (BioTech Life Science Dictionary)

Topic snapshot

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Updated
2026-10-18
Author
National Cancer Institute (source definition)
Reviewer
Automated source check (NCI definition)

What it is

A highly sensitive technique for detecting and measuring antigens or antibodies in a solution; the solution is run over a surface to which immobilized antibodies specific to the substance have been attached, and if the substance is present, it will bind to the antibody layer, and its presence is verified and visualized with an application of antibodies that have been tagged in some way. (BioTech Life Science Dictionary)

Source: National Cancer Institute, NCI Thesaurus 26.09d, C16553. NCI definition, reproduced without modification under CC BY 4.0.

Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.

Compliance note

Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.