National Cancer Institute (source definition)
Chromogenic In Situ Hybridization
A laboratory procedure that combines the sequence-specific detection aspects of FISH with the simple detection methods of immunohistochemistry. Briefly, sequence-specific probes are hybridized to cells fixed on slides and the sequestered probe is visualized by a peroxidase reaction. It has the advantage of being a less costly assay that does not require fluorescence microscopy with results that are stable over time.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-20
professional use
References
- NCI Thesaurus 26.09d — Chromogenic In Situ Hybridization (C40960)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C40960. NCI definition, reproduced without modification under CC BY 4.0.
- Diagnostic Utility of USP6 RNA Chromogenic In Situ Hybridization in the Differential Diagnosis of Nodular Fasciitis and Aneurysmal Bone Cyst. — Am J Surg Pathol, 2026-10-09 (PMID 42850764; literature)
- Punctate nuclear low-risk HPV chromogenic in situ hybridization in digital papillary adenocarcinoma: a practical tool for identifying HPV42 in routine pathology practice. — Histopathology, 2026-09-04 (PMID 42697604; literature)
- HER2 status in extrahepatic cholangiocarcinoma and gallbladder carcinoma: Concordance between immunohistochemistry and chromogenic in situ hybridization in 140 Cases. — Hum Pathol, 2026-08-14 (PMID 42595189; literature)
- USP6 RNA Chromogenic In Situ Hybridization Is Useful in the Diagnosis of Nodular Fasciitis and Aneurysmal Bone Cyst. — Mod Pathol, 2026-07-27 (PMID 42508574; literature)
- Validation of an automated chromogenic in situ hybridization protocol for detection of cytomegalovirus in formalin-fixed, paraffin-embedded renal graft biopsies. — J Bras Nefrol, 2026-07-01 (PMID 42047535; literature)
- Chromogenic in situ HCR as a targeted translational interface for routine molecular histology. — Histochem Cell Biol, 2026-06-08 (PMID 42257912; literature)
- Detection of human papillomavirus with low-risk chromogenic in situ hybridization probes in seborrheic keratosis-like lesions of the male genital tract. — Hum Pathol, 2026-03-10 (PMID 41812829; literature)
What it is
A laboratory procedure that combines the sequence-specific detection aspects of FISH with the simple detection methods of immunohistochemistry. Briefly, sequence-specific probes are hybridized to cells fixed on slides and the sequestered probe is visualized by a peroxidase reaction. It has the advantage of being a less costly assay that does not require fluorescence microscopy with results that are stable over time.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C40960. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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