National Cancer Institute (source definition)
Chromogenic In Situ Hybridization
A laboratory procedure that combines the sequence-specific detection aspects of FISH with the simple detection methods of immunohistochemistry. Briefly, sequence-specific probes are hybridized to cells fixed on slides and the sequestered probe is visualized by a peroxidase reaction. It has the advantage of being a less costly assay that does not require fluorescence microscopy with results that are stable over time.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-19
professional use
References
- NCI Thesaurus 26.09d — Chromogenic In Situ Hybridization (C40960)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C40960. NCI definition, reproduced without modification under CC BY 4.0.
- Diagnostic Utility of USP6 RNA Chromogenic In Situ Hybridization in the Differential Diagnosis of Nodular Fasciitis and Aneurysmal Bone Cyst. — Am J Surg Pathol, 2026-10-09 (PMID 42850764; literature)
- Punctate nuclear low-risk HPV chromogenic in situ hybridization in digital papillary adenocarcinoma: a practical tool for identifying HPV42 in routine pathology practice. — Histopathology, 2026-09-04 (PMID 42697604; literature)
- HER2 status in extrahepatic cholangiocarcinoma and gallbladder carcinoma: Concordance between immunohistochemistry and chromogenic in situ hybridization in 140 Cases. — Hum Pathol, 2026-08-14 (PMID 42595189; literature)
- USP6 RNA Chromogenic In Situ Hybridization Is Useful in the Diagnosis of Nodular Fasciitis and Aneurysmal Bone Cyst. — Mod Pathol, 2026-07-27 (PMID 42508574; literature)
What it is
A laboratory procedure that combines the sequence-specific detection aspects of FISH with the simple detection methods of immunohistochemistry. Briefly, sequence-specific probes are hybridized to cells fixed on slides and the sequestered probe is visualized by a peroxidase reaction. It has the advantage of being a less costly assay that does not require fluorescence microscopy with results that are stable over time.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C40960. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.