Technology
Whole Transcriptome Sequencing
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an individual.
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Technology
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an individual.
Glossary Term
The complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific cell.
Technology
Quantitative PCR: a method to measure very small amounts of RNA transcripts as cDNA targets. There are two strategies currently used, one is called Competitive RTPCR and the other is called Real-Time PCR.
Technology
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse transcriptase.
Technology
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Glossary Term
Single-stranded DNA that is complementary to messenger RNA or DNA that has been synthesized from messenger RNA by reverse transcriptase.
Article
Research record from Clin Microbiol Infect, 2026-03-28. PMID 41911972.
Glossary Term
An RNA-dependent DNA polymerase that is involved in the replication of retroviruses an in the maintenance of telomeres.
Glossary Term
A two-step setup in which RNA is first copied into complementary DNA by a reverse transcriptase and then amplified by PCR. Used for RNA viruses such as influenza, RSV, SARS-CoV-2, and HCV. In procurement documents, confirm whether "RT-PCR" refers to real-time (quantitative) or endpoint (qualitative) reading.
Glossary Term
The action of copying RNA into DNA by reverse transcriptase.
Indicators
This gene is involved in cell cycle regulation and telomere maintenance.