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A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.
Technology
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
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Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
Plan PCR throughput by daily sample volume, turnaround targets, staffing model and automation fit across low to high volume labs.
Article
Research record from Cancer Epidemiol, 2026-10-06. PMID 42837910.
Indicators
A family of globular proteins found in many plant and animal tissues that tend to bind a wide variety of ligands. Albumin is the main protein in blood plasma. Low serum levels occur in conditions associated with malnutrition, inflammation and liver and kidney diseases.
Tests
An albumin blood test measures the amount of albumin in your blood. Albumin is a protein made by your liver. Low albumin levels can be a sign of liver or kidney disease or another medical condition. High levels may be a sign of dehydration .
Tests
An AFP tumor marker test is a blood test that measures the level of AFP (alpha-fetoprotein) in a sample of your blood. AFP is a protein that the liver makes when its cells are growing and dividing to make new cells. AFP levels are normally high in a fetus, but after birth, they drop very low. Healthy children and adults who aren’t pregnant have very little AFP in their blood.
Article
Research record from Anal Chem, 2026-08-01. PMID 42610923.