Technology
Cell Culture
General term referring to the maintenance of cell strains or lines in the laboratory.
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Technology
General term referring to the maintenance of cell strains or lines in the laboratory.
Technology
Any of various processes to isolate one or more specific cell populations from a heterogeneous mixture of cells.
Technology
A molecular biology technique used to detect the interaction of a DNA binding protein with its cognate binding sequence. Labeled DNAs were reacted with crude cell extracts and the complexes are run through a non-denaturing polyacrylamide gel. The migration of the labeled DNA through the gel will be slower by being bound. This shift in electrophoretic mobility indicates functional binding between the protein and the DNA.
Technology
Selection and deposition of individual cells of a particular phenotype from a mixed population into a separate tube or tissue culture plate by the use of a fluorescence-activated cell sorter (FACS) and fluorescently-labeled antibodies specific for surface molecules on the cells to be sorted.
Technology
This is a method (named after the Danish bacteriologist, Hans Christian Gram) for staining and identifying bacteria. It is based on the differential retention of a crystal violet-iodine complex within the cell membrane. Gram-positive organisms will appear blue-black or purple while Gram-negative organisms will appear red.
Technology
An in vitro assay that is used to determine whether antibodies present in serum samples will prevent hemagglutination in a cell culture plate containing red blood cells and influenza virus.
Technology
The preparation, analysis, and interpretation of a karyotype, the representation of the chromosome set of a cell.
Technology
A high-resolution method used to isolate cells from their surrounding tissues, with the aid of a laser beam, under direct microscopic visualization. Specific cells can be isolated directly by cutting target cells away from unwanted cells, to obtain enriched cell populations.
Technology
An analysis done to detect monoallelic loss of genes or DNA regions in a diploid cell.
Technology
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow of patients who are to undergo autologous bone marrow transplantation.
Technology
A laboratory method in which the tissue from a histology slide is dissected under a microscope to procure specific cells or cell populations.
Technology
A serological test that measures the ability of antibodies in specific concentrations of serum to prevent the formation of plaques in a cell monolayer when combined with a viral suspension.