Technology
Nephelometry
A method to quantify the concentration of particulate matter suspended in a liquid or gas using a nephelometer.
Loading
Loading
Search products, companies, tests, and guides — go from query to quote request in one place.
Technology
A method to quantify the concentration of particulate matter suspended in a liquid or gas using a nephelometer.
Technology
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
Technology
Detection and measurement of the resonant spectra of molecular species in a tissue or sample.
Technology
A method for collecting tandem mass spectrometry data where an ion of a particular mass is selected in the first stage and a fragmented ion product is selected in the second mass spectrometer stage for detection. Parallel reaction monitoring (PRM)-based experiments are generally performed in a high-resolution hybrid quadrupole-Orbitrap (Q-OT) or time-of-flight mass spectrometer.
Technology
A simple non-quantitative form of interference microscopy of great utility in visualising live cells. Small differences in optical path length due to differences in refractive index and thickness of structures are visualised as differences in light intensity.
Technology
A type of spectroscopy that utilizes a laser beam to irradiate a sample containing particles in suspension, resulting in light scattering. Rapid fluctuations in scattering intensity, around a mean value at a certain angle, occur because of particle diffusion and are dependent upon on particle size. The calculated correlation function yields a diffusion coefficient, for a given temperature and viscosity, which can be used to calculate particle size.
Technology
A serological test that measures the ability of antibodies in specific concentrations of serum to prevent the formation of plaques in a cell monolayer when combined with a viral suspension.
Technology
Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.
Technology
A method for amplifying a DNA base sequence using multiple rounds of heat denaturation of the DNA and annealing of oligonucleotide primers complementary to flanking regions in the presence of a heat-stable polymerase. This results in duplication of the targeted DNA region. Newly synthesized DNA strands can subsequently serve as additional templates for the same primer sequences, so that successive rounds of primer annealing, strand elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR also can be used to detect the existence of the defined sequence in a DNA sample.
Technology
Analysis that looks for differences in the lengths of microsatellite repeats between alleles. Polymorphic microsatellites are present in the genome every few thousand base pairs and can function as molecular markers.
Technology
An electroanalytical technique in which the electrical potential of a solution is measured to determine the composition of the sample, by comparing the constant potential of the reference electrode with the actual potential of the indicator electrode.
Technology
Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.