National Cancer Institute (source definition)
Nested PCR
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-26
professional use
References
- NCI Thesaurus 26.09d — Nested PCR (C127880)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C127880. NCI definition, reproduced without modification under CC BY 4.0.
- Semi-nested PCR for molecular identification of snake species from human snakebite wounds: recovery of snake DNA where conventional PCR fails. — Toxicon, 2026-09-28 (PMID 42805463; literature)
- Pan filarial-nested PCR with gelified tubes: a new approach towards point-of-care testing for human filariasisFIRST REVIEW ROUND - REVIEWERS' COMMENTSREVIEWERS' COMMENTS. — Mem Inst Oswaldo Cruz, 2026-09-25 (PMID 42820526; literature)
- Comparison of mtLSU-rRNA gene-based real-time PCR and nested PCR for the diagnosis of Pneumocystis jirovecii pneumonia in HIV-negative patients. — Diagn Microbiol Infect Dis, 2026-10-18 (PMID 42925658; literature)
What it is
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C127880. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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