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Technology
A highly sensitive technique for detecting and measuring antigens or antibodies in a solution; the solution is run over a surface to which immobilized antibodies specific to the substance have been attached, and if the substance is present, it will bind to the antibody layer, and its presence is verified and visualized with an application of antibodies that have been tagged in some way. (BioTech Life Science Dictionary)
Technology
Any of several immunoassay methods that use an enzyme covalently linked to an antigen or antibody as a label; the most common types are enzyme-linked immunosorbent assay (ELISA) and enzyme-multiplied immunoassay technique (EMIT).
Technology
A type of homogenous immunoassay in which sample is applied to a reagent mixture of enzyme-labeled ligand, antibody, and substrate. Substrate binding to enzyme leads to inactivation and unlabeled-ligand in the sample can be quantified by colorimetric analysis.
Technology
A method to determine microbial susceptibility to antibiotics in which a plastic strip impregnated with the antibiotic of interest is placed on an agar plate that has been inoculated with bacteria. The antibiotic diffuses from the strip producing a concentration gradient of drug on the agar. The point at which the elliptical shaped area of growth inhibition meets the strip is the minimum inhibitory concentration of the drug of interest.
Technology
The process of passing a liquid or gas through a filter.
Technology
Selection and deposition of individual cells of a particular phenotype from a mixed population into a separate tube or tissue culture plate by the use of a fluorescence-activated cell sorter (FACS) and fluorescently-labeled antibodies specific for surface molecules on the cells to be sorted.
Technology
A technique in which samples that either contain fluorescent compounds or are stained with fluorescent dyes are exposed to a wavelength of light that excites the fluorophore, allowing for an image to be captured by a microscope.
Technology
An immunoassay in which a fluorescent tracer is used to detect an analyte of interest by triggering the emission of photons when subjected to polarized light.
Technology
A process to measure the proximity of two fluorochromes whereby the excitation energy of one fluorochrome is released and absorbed by the second fluorochrome. It is used to study interactions and structure of macromolecules. (NCI)
Technology
Fluorescent spectroscopy or fluorometry is a type of electromagnetic spectroscopy used for analyzing fluorescent spectra. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light of a lower energy, typically, but not necessarily, visible light.
Technology
An immunological procedure in which the antibodies are coupled with molecules which fluoresce under ultra violet (UV) light. This makes them particularly suitable for detection of specific antigens in tissues or on cells.
Technology
A chromatographic technique in which the stationary phase is solid while the mobile phase is gaseous samples. The gaseous samples are separated based on their different adsorption ability to the solid phase.
