Glossary Term
Read Depth
The number of times a particular locus (site, nucleotide, amplicon, region) was sequenced.
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Glossary Term
The number of times a particular locus (site, nucleotide, amplicon, region) was sequenced.
Glossary Term
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
Glossary Term
One of the three possible ways of reading a nucleotide sequence. As the genetic code is read in nonoverlapping triplets (codons) there are three possible ways of translating a sequence of nucleotides into a protein, each with a different starting point.
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Any natural or synthetic substance used in a chemical or biological reaction in order to produce, identify, or measure another substance.
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An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
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A set of values of particular type of measurement that is used to interpret corresponding observation results.
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The ability to yield similar results given a set of constraints.
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Enzymes that exhibit endonuclease activity at a specific short nucleotide sequence to cleave strands of DNA.
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A sequence in DNA that can be recognized by a specific restriction enzyme.
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A study in which participants with known outcomes are assessed based on data collected before the study.
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An RNA-dependent DNA polymerase that is involved in the replication of retroviruses an in the maintenance of telomeres.
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The action of copying RNA into DNA by reverse transcriptase.