Glossary Term
Reagent
Any natural or synthetic substance used in a chemical or biological reaction in order to produce, identify, or measure another substance.
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Glossary Term
Any natural or synthetic substance used in a chemical or biological reaction in order to produce, identify, or measure another substance.
Glossary Term
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
Glossary Term
A PCR method that measures amplification as it happens using fluorescent reporters, so target quantity is estimated from the amplification curve rather than by post-run handling. qPCR is the workhorse format for viral load, pathogen detection, and GMO or food testing workflows.
Glossary Term
Enzymes that exhibit endonuclease activity at a specific short nucleotide sequence to cleave strands of DNA.
Glossary Term
A sequence in DNA that can be recognized by a specific restriction enzyme.
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An RNA-dependent DNA polymerase that is involved in the replication of retroviruses an in the maintenance of telomeres.
Glossary Term
A two-step setup in which RNA is first copied into complementary DNA by a reverse transcriptase and then amplified by PCR. Used for RNA viruses such as influenza, RSV, SARS-CoV-2, and HCV. In procurement documents, confirm whether "RT-PCR" refers to real-time (quantitative) or endpoint (qualitative) reading.
Glossary Term
The action of copying RNA into DNA by reverse transcriptase.
Glossary Term
Ribonuclease inhibitor (461 aa, ~50 kDa) is encoded by the human RNH1 gene. This protein plays a role in the inhibition of ribonucleases.
Glossary Term
Single-stranded long chain of nucleotides containing ribose. It is the end product of DNA transcription by the enzyme RNA polymerase. It is essential in protein synthesis.
Glossary Term
An RNA molecule that does not contain protein-coding sequence information and constitutes the majority of the cellular RNA (~80%). This nucleic acid associates with multiple proteins to form ribosomes, which are the organelles that drive mRNA-directed protein synthesis.
Glossary Term
Removal of introns from mRNA precursors and the reattachment or annealing of exons.