Technology
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Diagnostic Methods
Technology
An electron microscopy imaging technique that is utilized to examine structural components of a sample by passing electrons through the specimen.
Diagnostic Methods
Technology
A method to quantify the opaqueness of a liquid using a turbidimeter, by measuring the decrease in light intensity due to the scattering effect of suspended particles in the liquid sample.
Diagnostic Methods
Technology
A laboratory procedure in which proteins are first separated across a gel, usually polyacrylamide, according to their isoelectric point, and then separated in a perpendicular direction on the basis of their molecular weight.
Diagnostic Methods
Technology
Technology that detects differences between an individual's DNA sequence and a reference DNA sequence.
Diagnostic Methods
Technology
A diagnostic procedure to identify and characterize a viral organism.
Diagnostic Methods
Technology
A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Diagnostic Methods
Technology
A procedure that can determine the DNA sequence for all of the exons in an individual.
Diagnostic Methods
Technology
Any technique designed to amplify a limited genomic DNA sample so as to generate a new sample that is indistinguishable from the original but with a higher DNA concentration.
Diagnostic Methods
Technology
A procedure that can determine the DNA sequence for nearly the entire genome of an individual.
Diagnostic Methods
Technology
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an individual.
Diagnostic Methods