National Cancer Institute (source definition)
Western Blotting
A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Review and references
Automated source check (NCI definition)
2026-10-18
2026-10-26
professional use
References
- NCI Thesaurus 26.09d — Western Blotting (C16357)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C16357. NCI definition, reproduced without modification under CC BY 4.0.
- Cross-species evaluation of commercial primary antibodies by Western blotting for immunohistochemical protein detection in mouse, rat, and human brain tissue. — J Neurosci Methods, 2026-10-23 (PMID 42918555; literature)
What it is
A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C16357. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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