Glossary Term
Multiplex PCR
Abbreviation: PCR
PCR TermsEN
Definition
Amplification of several targets in a single reaction using multiple primer pairs, each distinguishable by fluorescent channel or melting profile. Multiplexing raises information per sample but makes channel allocation, dye compatibility, and competition between targets key selection criteria.
Extended Notes
Multiplex capability should be assessed together with menu fit, control design, and interpretation complexity.
Also Known As
PCR
Related Terms
References
- Highly sensitive detection and identification of six viruses in shrimps by universal primer based multiplex PCR. — J Invertebr Pathol, 2026-09-25 (PMID 42790584; literature)
- Respiratory viral and bacterial co-infections in adults in acute care: A multiplex PCR-based study. — PLoS One, 2026-09-25 (PMID 42789570; literature)
- Development and evaluation of a multiplex PCR for simultaneous detection of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Burkholderia pseudomallei in clinical specimens. — PLoS Negl Trop Dis, 2026-09-24 (PMID 42784601; literature)
- Epidemiology of Lower Respiratory Tract Infections in Hospitalized Patients Using Multiplex PCR in Central Greece. — Acta Med Acad, 2026-09-23 (PMID 42788274; literature)
- Mycoplasma pneumoniae-associated reactive infectious mucocutaneous eruption (RIME) confirmed by multiplex PCR: a case-based review. — Ir J Med Sci, 2026-09-22 (PMID 42771254; literature)
- High-risk Human Papillomavirus Genotypes in Women Undergoing Cervical Screening in Leshan, China: Real-time and Nested Multiplex PCR. — J Vis Exp, 2026-10-13 (PMID 42872767; literature)
- Species-specific detection of Capnocytophaga in domestic dogs (Canis lupus familiaris) and domestic cats (Felis catus) using a two reaction end-point multiplex PCR assay. — J Microbiol Methods, 2026-10-12 (PMID 42863575; literature)
- Multiplex digital PCR for rapid quantitative detection and dynamic monitoring of respiratory pathogens in bronchoalveolar lavage fluid. — Analyst, 2026-10-13 (PMID 42606556; literature)
- Development of a multiplex PCR for detection of pathogenic Mycobacterium orygis in cattle tissues harboring tuberculous-like lesions: a pilot study. — Trop Anim Health Prod, 2026-10-10 (PMID 42859325; literature)