Glossary Term
Short Tandem Repeat
Area of repetitive DNA within the genome consisting of multiple, end-to-end copies of a short DNA sequence usually comprised of di-, tri-, or tetranucleotide repeat units.
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Glossary Term
Area of repetitive DNA within the genome consisting of multiple, end-to-end copies of a short DNA sequence usually comprised of di-, tri-, or tetranucleotide repeat units.
Glossary Term
Initiation of DNA base excision repair (BER) involves a DNA glycosylase that binds the altered deoxynucleoside in an extrahelical position and catalyzes cleavage of the base-sugar bond. Subsequently, an AP endonuclease or AP lyase activity incises the abasic site, followed by short-patch gap-filling, excision of the base-free sugar-phosphate residue, and ligation.
Glossary Term
Short DNA oligonucleotide chains used to prime DNA (and in some cases RNA) synthesis.
Glossary Term
Complementary precision measures: repeatability is variation under the same conditions (same operator, equipment, short interval); reproducibility is variation across conditions — different days, operators, lots, or laboratories.
Glossary Term
Enzymes that exhibit endonuclease activity at a specific short nucleotide sequence to cleave strands of DNA.
Glossary Term
A class of short adaptor RNAs that transfers a specific amino acid to a growing polypeptide chain at the ribosomal site of protein synthesis during translation. Each transfer RNA comprises a three-nucleotide sequence that is complementary to the triplet nucleotide coding sequence of an mRNA, conferring specificity to the amino acid being incorporated into the assembling protein.
Glossary Term
Short random nucleotide sequences that are ligated to each primer molecule during amplification of a nucleotide sample during library preparation to act as a unique tag. Their use helps to identify PCR duplicates in sequencing data.