Glossary Term
Sequencing Library
A collection of double stranded DNA fragments flanked by oligonucleotide sequence adapters to enable their analysis by high-throughput sequencing.
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Glossary Term
A collection of double stranded DNA fragments flanked by oligonucleotide sequence adapters to enable their analysis by high-throughput sequencing.
Glossary Term
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Glossary Term
Technologies that facilitate the rapid determination of the nucleotide sequence of large numbers of strands or segments of DNA or RNA.
Glossary Term
The global analysis of cellular proteins. Proteomics uses a combination of sophisticated techniques including two-dimensional (2D) gel electrophoresis, image analysis, mass spectrometry, amino acid sequencing, and bio-informatics to resolve comprehensively, to quantify, and to characterize proteins. The application of proteomics provides major opportunities to elucidate disease mechanisms and to identify new diagnostic markers and therapeutic targets.
Glossary Term
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
Glossary Term
Short random nucleotide sequences that are ligated to each primer molecule during amplification of a nucleotide sample during library preparation to act as a unique tag. Their use helps to identify PCR duplicates in sequencing data.
Glossary Term
A procedure that can determine the DNA sequence for nearly the entire genome of an individual.