Technology
Cytogenetic Analysis
Techniques for analysis of chromosomal and subchromosomal properties and structures, such as those to diagnose, classify, screen for, or manage genetic diseases and abnormalities.
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Technology
Techniques for analysis of chromosomal and subchromosomal properties and structures, such as those to diagnose, classify, screen for, or manage genetic diseases and abnormalities.
Technology
A procedure used in mass spectrometry analysis. Means of delivering nonvolatile analytes into the gas phase for mass analysis. Best used on molecules which are charged in solution or readily accept a charge. Particularly well suited to the analysis of biomolecules. Can be used to probe tertiary structure and non-covalent interactions (from NCSU Mass Spectrometry Facility web site)
Technology
A type of homogenous immunoassay in which sample is applied to a reagent mixture of enzyme-labeled ligand, antibody, and substrate. Substrate binding to enzyme leads to inactivation and unlabeled-ligand in the sample can be quantified by colorimetric analysis.
Technology
The process of measuring the extent of crossovers between chromosomes as a gauge of the distance between genes.
Technology
The preparation, analysis, and interpretation of a karyotype, the representation of the chromosome set of a cell.
Technology
An analysis done to detect monoallelic loss of genes or DNA regions in a diploid cell.
Technology
An analysis of DNA or protein to detect the presence and/or location of methyl groups.
Technology
Analysis using microarray technology (e.g., cDNA arrays to see gene expression or protein microarrays to profile the pattern of proteins).
Technology
A laboratory procedure to detect microsatellite instability.
Technology
Analysis that looks for differences in the lengths of microsatellite repeats between alleles. Polymorphic microsatellites are present in the genome every few thousand base pairs and can function as molecular markers.
Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A procedure used to demonstrate nucleotide sequence variations that occur in the same region of DNA between individuals within a species, or between alleles in an individual to detect mutations in various genes, such as oncogenes, tumor suppressor genes, or genes responsible for genetic diseases. The method detects different conformations adopted by partially denatured DNA fragments resulting from their specific nucleotide sequences under denaturing conditions. Denatured single-stranded DNA fragments are allowed to partially renature without formation of double-stranded DNA. The particular conformations adopted by each of the renatured fragments depend on each of their specific nucleotide sequences. The fragments are run on polyacrylamide gels to detect subtle changes in migration due to their particular secondary structures. Resulting fragment band in the gel will misalign if any point mutations are present to alter the conformation.