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Glossary Term
A sequence of single-stranded RNA, that is 20-25 nucleotides in length, which is transcribed from DNA but is not translated into a protein. This nucleic acid may regulate the transcription or translation of other genes.
Glossary Term
Technologies that facilitate the rapid determination of the nucleotide sequence of large numbers of strands or segments of DNA or RNA.
Glossary Term
A sequence of nucleotides in that contains no termination codons and so can potentially translate as a polypeptide chain.
Glossary Term
A score reported on a logarithmic scale representing the probability that a nucleotide base was incorrectly called by the base caller.
Glossary Term
A small-scale mutation in which a single nucleotide is exchanged for another. Mutagenic chemicals and mistakes in DNA replication may result in point mutations.
Glossary Term
Encoded by Polymerase Genes, Polymerase enzymes catalyze polymerization reactions, especially of nucleotides to polynucleotides. (NCI)
Glossary Term
The number of times a particular locus (site, nucleotide, amplicon, region) was sequenced.
Glossary Term
The number of nucleotides successfully ordered from each side of a nucleic acid fragment obtained after the completion of a sequencing process.
Glossary Term
One of the three possible ways of reading a nucleotide sequence. As the genetic code is read in nonoverlapping triplets (codons) there are three possible ways of translating a sequence of nucleotides into a protein, each with a different starting point.
Glossary Term
Enzymes that exhibit endonuclease activity at a specific short nucleotide sequence to cleave strands of DNA.
Glossary Term
Single-stranded long chain of nucleotides containing ribose. It is the end product of DNA transcription by the enzyme RNA polymerase. It is essential in protein synthesis.
Glossary Term
A long linear single-stranded polymer formed from nucleotides attached to a deoxyribose backbone.
