Artículo
The diagnostic potential of combined quantitative polymerase chain reaction and next-generation sequencing using the same primers for periprosthetic joint infection.
Research record from Microbiol Spectr, 2026-07-28. PMID 42517638.
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Artículo
Research record from Microbiol Spectr, 2026-07-28. PMID 42517638.
Technology
A type of nucleic acid amplification test in which sample DNA is exposed to a single temperature in the presence of multiple sets of primers and a polymerase with replication and high strand displacement activity. This test negates the need for thermal cyclers and may be a low cost alternative to PCR.
Technology
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
Technology
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
Technology
An isothermal, in vitro nucleic acid amplification method that allows primer-directed amplification of a target sequence. It utilizes a primer that can be nicked to create a polymerase start site and a DNA polymerase that will displace a downstream strand as it tracks along the template.
Technology
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
Pruebas
The determination of the hepatitis B viral load in a sample using polymerase chain reaction.
Pruebas
The determination of the hepatitis C viral load in a sample using polymerase chain reaction.
Pruebas
The determination of the HIV viral load in a specimen using polymerase chain reaction.
Glossary Term
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target within the first run product.
Pruebas
PCR (polymerase chain reaction) tests are a reliable and accurate way to diagnose certain infectious diseases , some types of cancer , and certain genetic changes. The tests check for small amounts of genetic material of a pathogen (disease-causing organism) or abnormal cells in a sample of your blood, saliva, mucus, or tissue. The genetic material could be:
Glossary Term
A DNA sequence at which RNA polymerase binds and initiates transcription.