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Technology
An immunological procedure in which antibodies are coupled with a peroxidase molecule. Following localization via the antibody, the peroxidase is reacted with a corresponding substrate to produce a product which can be seen with a light microscope.
Technology
Antigen aggregation with antibody, in the right ratios, to cause precipitation.
Technology
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow of patients who are to undergo autologous bone marrow transplantation.
Technology
A type of enzyme immunoassay that uses antigen-coated microparticles as a substrate for antibody binding.
Technology
A quantitative technique used to detect and estimate the concentration of an antigen by measuring the diameter of the ring of precipitation (precipitin ring), which is formed as a result of the interaction of the antigen and an antibody at optimal concentration.
Technology
Radioimmunoassay (RIA) is an immunological technique, which quantitatively determines antigen and antibody concentrations, using a radioactively labeled substance (radioligand), either directly or indirectly.
Technology
A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Technology
Selection and deposition of individual cells of a particular phenotype from a mixed population into a separate tube or tissue culture plate by the use of a fluorescence-activated cell sorter (FACS) and fluorescently-labeled antibodies specific for surface molecules on the cells to be sorted.
Technology
An in vitro assay that is used to determine whether antibodies present in serum samples will prevent hemagglutination in a cell culture plate containing red blood cells and influenza virus.
Technology
Affinity chromatography using monoclonal or polyclonal antibodies, or (less commonly) antigens, as the immobilized binding phase.
Technology
A method for the detection of proteins or antigens on samples of cells that are grown in monolayers or in suspension and deposited on slides using antibodies or other biomolecules that bind the target and can be visualized through a chemical process.
Technology
Laboratory test involving interaction of antigens with specific antibodies.
