Artículo
Noninvasive Detection of Bladder Cancer via Urinary DNA Mutation and Methylation Profiling Using Modified Real-Time Quantitative PCR.
Research record from J Mol Diagn, 2026-07-21. PMID 42480851.
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Artículo
Research record from J Mol Diagn, 2026-07-21. PMID 42480851.
Artículo
Research record from Int J Dermatol, 2026-07-18. PMID 42470253.
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Research record from Small, 2026-06-12. PMID 42283595.
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Research record from Sci Rep, 2026-04-08. PMID 41951723.
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Research record from Toxicon, 2026-09-28. PMID 42805463.
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Research record from Anal Chim Acta, 2026-05-05. PMID 42191257.
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Research record from J Mol Diagn, 2026-09-11. PMID 42727688.
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Research record from Front Med (Lausanne), 2026-09-07. PMID 42769177.
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Research record from J Gynecol Oncol, 2026-04-22. PMID 42108733.
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Research record from Eur J Clin Microbiol Infect Dis, 2026-03-06. PMID 41790343.
Technology
A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.
Technology
A molecular cytogenetic method combining chromosomal comparative genomic hybridization technique and microarray to detect chromosomal aberrations and changes in DNA copy numbers in the genome, using oligonucleotides immobilized on a solid support as targets for hybridization.