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Technology
An immunological procedure in which the antibodies are coupled with molecules which fluoresce under ultra violet (UV) light. This makes them particularly suitable for detection of specific antigens in tissues or on cells.
Technology
Affinity chromatography using monoclonal or polyclonal antibodies, or (less commonly) antigens, as the immobilized binding phase.
Technology
A type of chromatography assay in which an antibody-conjugate is passed over an immobilized antigen, giving a visual indication of positive or negative result.
Technology
A method for the detection of proteins or antigens on samples of cells that are grown in monolayers or in suspension and deposited on slides using antibodies or other biomolecules that bind the target and can be visualized through a chemical process.
Technology
A quantitative technique used to detect and estimate the concentration of an antigen by measuring the precipitation which is formed as a result of the interaction of the antigen and an antibody at optimal concentration.
Technology
An enhancement of immunoelectrophoresis in which a series of post-electrophoretic gel slabs are layered with cellulose-acetate gels saturated with specific antibodies. The resulting antigen-antibody complexes fixed on the second gel may then be stained, allowing sensitive and specific qualitative visual identification of paraproteins by electrophoretic position. It can be used to identify specific proteins in the blood or urine. It has greatest application in the identification and monitoring of monoclonal proteins that are produced in conditions like Waldenstom's macroglobulinaemia and multiple myeloma.
Technology
Immunohistochemical staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
Technology
Laboratory test involving interaction of antigens with specific antibodies.
Technology
Antigen aggregation with antibody, in the right ratios, to cause precipitation.
Technology
A flow cytometry technique that utilizes mass spectrometry to detect antibodies labeled with heavy metal ion tags that are bound to antigens of interest on single cells.
Technology
A type of enzyme immunoassay that uses antigen-coated microparticles as a substrate for antibody binding.
Technology
A quantitative technique used to detect and estimate the concentration of an antigen by measuring the diameter of the ring of precipitation (precipitin ring), which is formed as a result of the interaction of the antigen and an antibody at optimal concentration.
