Test guide

Indirect Bilirubin Measurement

Bilirubin is in the insoluble form, unconjugated bilirubin (indirect bilirubin).The non-water soluble, free bilirubin does not react with sulfanilic acid at acidic pH to produce a red colored complex until an accelearator, alcohol, is added to the solution to perform a quantitative measurement of unconjugated bilirubin levels.

For professionalsReviewed 2026-10-20

What it is for

Source: National Cancer Institute, NCI Thesaurus 26.09d, C64483. Unmodified NCI definition, CC BY 4.0. Source identity and classification checked automatically on 2026-10-20.

Limitations

Terminology reference for a laboratory procedure. The source definition does not establish routine clinical availability, validated specimen types, reference intervals, product authorization or suitability for an individual. Consult the performing laboratory for its validated method and interpretation.

Also known as

  • Indirect Bilirubin Measurement
  • Unconjugated Bilirubin Measurement

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