National Cancer Institute (source definition)
Isoelectric Focusing
A type of gel electrophoresis in which charged molecules travel through a pH gradient. The molecules will come to stop at the point where the net charge on the molecule is equal to that of the surrounding gel.
Review and references
Automated source check (NCI definition)
2026-10-19
2026-10-19
professional use
- NCI Thesaurus 26.09d — Isoelectric Focusing (C16758)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C16758. NCI definition, reproduced without modification under CC BY 4.0.
- Visualized separation of exosomes in urine using carrier ampholyte-free free-flow isoelectric focusing electrophoresis. — J Chromatogr A, 2026-09-28 (PMID 42826545; literature)
- Investigation of lipid nanoparticle stability and integrity under isoelectric focusing stress conditions using taylor dispersion analysis. — Anal Chim Acta, 2026-07-25 (PMID 42702450; literature)
- A Novel Fully Automatic Free-Flow Isoelectric Focusing System and Its Application in the Large-Scale Isolation of Milk Exosomes. — Electrophoresis, 2026-07-10 (PMID 42429349; literature)
- Dual-modal deep learning model with microstrip isoelectric focusing and imaging strategy for feed classification. — Anal Chim Acta, 2026-05-25 (PMID 42242815; literature)
What it is
A type of gel electrophoresis in which charged molecules travel through a pH gradient. The molecules will come to stop at the point where the net charge on the molecule is equal to that of the surrounding gel.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C16758. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.
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