National Cancer Institute (source definition)
ChIP-Seq
A molecular genetic technique that combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to map the binding sites of DNA-associated proteins in a sample of cells. First, crosslinked protein-DNA complexes are isolated using ChIP. Next, the crosslinks are broken, the proteins are removed and the purified DNA is modified with adaptor oligonucleotides to facilitate massively parallel DNA sequencing. Following sequencing, the DNA sequences that are obtained can be mapped to their genomic locations.
Review and references
Automated source check (NCI definition)
2026-10-19
2026-10-19
professional use
References
- NCI Thesaurus 26.09d — ChIP-Seq (C106049)
- Source: National Cancer Institute, NCI Thesaurus 26.09d, C106049. NCI definition, reproduced without modification under CC BY 4.0.
- Optimized Genome-Wide Profiling of Centromeric Chromatin Complexes by ChIP-seq. — Curr Protoc, 2026-10-01 (PMID 42841660; literature)
- Detecting Meiotic Crossing-Overs in Maize Using Chromatin Immunoprecipitation-Sequencing (ChIP-seq). — Cold Spring Harb Protoc, 2026-10-01 (PMID 42722403; literature)
What it is
A molecular genetic technique that combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to map the binding sites of DNA-associated proteins in a sample of cells. First, crosslinked protein-DNA complexes are isolated using ChIP. Next, the crosslinks are broken, the proteins are removed and the purified DNA is modified with adaptor oligonucleotides to facilitate massively parallel DNA sequencing. Following sequencing, the DNA sequences that are obtained can be mapped to their genomic locations.
Source: National Cancer Institute, NCI Thesaurus 26.09d, C106049. NCI definition, reproduced without modification under CC BY 4.0.
Source identity and definition checked automatically on 2026-10-18. No individual clinical interpretation or human reviewer is asserted.
Compliance note
Reference intervals and interpretation depend on the laboratory, specimen, method and clinical context. This source definition is educational information; it does not establish a diagnosis, product approval or suitability for an individual.