Technology
A light microscopic technique in which the light that does not come into contact with the structures or details of interest is subtracted from the ocular image. This yields an image in which the structures or details are alight while the areas where the structures or details are absent are dark.
Diagnostic Methods
Technology
A chromatographic technique in which the stationary phase is solid while the mobile phase is gaseous samples. The gaseous samples are separated based on their different adsorption ability to the solid phase.
Diagnostic Methods
Technology
This is a method (named after the Danish bacteriologist, Hans Christian Gram) for staining and identifying bacteria. It is based on the differential retention of a crystal violet-iodine complex within the cell membrane. Gram-positive organisms will appear blue-black or purple while Gram-negative organisms will appear red.
Diagnostic Methods
Technology
Mass spectrometers coupled in series. The targeted compound is selectively ionized, and its characteristic ions are separated from others in the mixture in the first mass spectrometer. The selected primary ions are then decomposed by collision, and from the resulting products the final mass analyzer selects secondary ions characteristic of the targeted compound. Tandem mass spectrometry can achieve specificities and sensitivities equivalent of those of methods such as radioimmunoassay and gas chromatography/mass spectrometry, while performing analyses in much shorter times.
Diagnostic Methods