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Glossary Term
Enzymatic (e.g. dUTP/UNG) and physical measures that stop amplified DNA from contaminating later runs. Labs with high daily volumes usually require dUTP/UNG chemistry in purchased assays; confirm this when comparing kits from different manufacturers.
Glossary Term
Fluorescence is a luminescence (i.e., optical phenomenon) in cold bodies, in which a molecule absorbs a high-energy photon, and re-emits it as a lower-energy (longer-wavelength) photon. The energy difference between the absorbed and emitted photons ends up as molecular vibrations (heat).
Glossary Term
Placeholder definition before migration.
Glossary Term
A purification method in which nucleic acids bind to paramagnetic beads and are collected with a magnet through wash steps. It automates well, which is why most high-throughput extraction platforms are bead-based; compare deck footprint and sample capacity when shortlisting systems.
Glossary Term
A collection of double stranded DNA fragments flanked by oligonucleotide sequence adapters to enable their analysis by high-throughput sequencing.
