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Technology
Analysis that looks for differences in the lengths of microsatellite repeats between alleles. Polymorphic microsatellites are present in the genome every few thousand base pairs and can function as molecular markers.
Technology
An electroanalytical technique in which the electrical potential of a solution is measured to determine the composition of the sample, by comparing the constant potential of the reference electrode with the actual potential of the indicator electrode.
Technology
The process of determining the sequence of amino acids within a protein molecule.
Technology
Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.
Technology
A DNA sequencing method that uses a "sequencing by synthesis" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.
Technology
Quantitative PCR: a method to measure very small amounts of RNA transcripts as cDNA targets. There are two strategies currently used, one is called Competitive RTPCR and the other is called Real-Time PCR.
Technology
A quantitative technique used to detect and estimate the concentration of an antigen by measuring the diameter of the ring of precipitation (precipitin ring), which is formed as a result of the interaction of the antigen and an antibody at optimal concentration.
Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3'- or 5'-end of the mRNA.
Technology
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
Technology
A method to measure the refractive index (propensity for light to bend) of a sample.
Technology
Variation occurring within a species in the length of DNA fragments generated by a specific endonuclease. Such variations are generated by mutations that create or abolish recognition sites for these enzymes.
