Technology
Microsatellite Instability Analysis
A laboratory procedure to detect microsatellite instability.
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Technology
A laboratory procedure to detect microsatellite instability.
Technology
A common molecular biology procedure for detection of RNA molecules. RNA extracted from a sample was denatured and separated by slab gel electrophoresis, then blotted on to a nitrocellulose or nylon sheet and hybridized with labeled DNA or RNA that is complementary to the desired RNA, whose presence is subsequently indicated by autoradiography or other visualization techniques.
Technology
Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.
Company
Promega Corporation is an IVD supplier based in United States, with a source-linked product portfolio. Molecular Diagnostics. Coverage spans Molecular Assays, Instruments | Gastrointestinal.
Technology
Separation of protein fractions by precipitation, chromatography, or other methods.
Technology
The process of determining the sequence of amino acids within a protein molecule.
Technology
Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.
Technology
Emission of electromagnetic energy with a shorter frequency (longer wavelength) than that of the incident monochromatic light. Arises from the low probability absorption of quanta with a higher energy than that required for a transition: the difference in energy is emitted as a lower frequency (energy) photon. Allows analysis of vibrational and rotational energy levels using visible incident light.
Technology
A procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown sequences at either the 3'- or 5'-end of the mRNA.
Technology
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the starting amount of template in the reaction.
Technology
A method to measure the refractive index (propensity for light to bend) of a sample.
Technology
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse transcriptase.