Technology
Cytochemistry
Branch of histochemistry associated with the localization of cellular components by specific staining methods, as for example the localization of acid phosphatases by the Gomori method.
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Technology
Branch of histochemistry associated with the localization of cellular components by specific staining methods, as for example the localization of acid phosphatases by the Gomori method.
Technology
The light microscopic study of normal and abnormal cells in fine needle aspirates (FNAs), body cavity fluids, and smears.
Technology
A light microscopic technique in which the light that does not come into contact with the structures or details of interest is subtracted from the ocular image. This yields an image in which the structures or details are alight while the areas where the structures or details are absent are dark.
Technology
A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.
Technology
A type of polymerase chain reaction technique in which the sample is fractionated, within which individual PCR reactions occur in each fraction.
Technology
A measurement of the antibody or complement-coated erythrocytes in a blood specimen in vivo.
Technology
Any method that extracts and purifies DNA from biological samples, which can include the proper handling of biological samples prior to nucleic acid isolation, the separation of DNA from the rest of the components in the samples, and the storage of the DNA isolates.
Technology
The determination of the sequence of purines and pyrimidines in deoxyribonucleic acid (DNA).
Technology
A method in which electromagnetic radiation, in the form of light emission, is generated from an electrochemical reaction in a solution due to the formation of antigen-antibody complexes.
Technology
Any form of microscopy in which the interactions of electrons with the specimens are used to provide information about the final structure of that specimen.
Technology
A molecular biology technique used to detect the interaction of a DNA binding protein with its cognate binding sequence. Labeled DNAs were reacted with crude cell extracts and the complexes are run through a non-denaturing polyacrylamide gel. The migration of the labeled DNA through the gel will be slower by being bound. This shift in electrophoretic mobility indicates functional binding between the protein and the DNA.
Technology
A procedure used in mass spectrometry analysis. Means of delivering nonvolatile analytes into the gas phase for mass analysis. Best used on molecules which are charged in solution or readily accept a charge. Particularly well suited to the analysis of biomolecules. Can be used to probe tertiary structure and non-covalent interactions (from NCSU Mass Spectrometry Facility web site)