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Technology
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA strand into a DNA complement is termed reverse transcription and is done by the enzyme reverse transcriptase.
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Technology
The process of arranging protein, DNA or RNA sequences to identify regions with similar sequences that may elucidate functional, structural, or evolutionary relationships between the sequences.
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Technology
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an amplified nucleotide sample that was derived from a single cell.
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Technology
A technique used to selectively replicate nucleic acid sequences from RNA templates using reverse transcriptase and RNA polymerase. Reverse transcriptase generates cDNA from the region of interest using primers and then RNA polymerase generates copies of the original RNA template.
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Technology
A procedure that can determine the nucleotide sequence for all of the RNA transcripts in an individual.
