Technology
Protein Sequencing
The process of determining the sequence of amino acids within a protein molecule.
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Technology
The process of determining the sequence of amino acids within a protein molecule.
Technology
Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.
Technology
The process of arranging protein, DNA or RNA sequences to identify regions with similar sequences that may elucidate functional, structural, or evolutionary relationships between the sequences.
Technology
Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis is a procedure for estimating molecular weights of polypeptides, and uses SDS, a detergent that dissociates and unfolds oligomeric proteins into its subunits. The SDS binds to the polypeptides to form complexes with fairly constant charge to mass ratios. The electrophoretic migration rate through a gel is therefore determined only by the size of the complexes.
Technology
A laboratory procedure in which proteins are first separated across a gel, usually polyacrylamide, according to their isoelectric point, and then separated in a perpendicular direction on the basis of their molecular weight.
Technology
A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.